Smorgasbord of Chemical Challenges for a Cardiac Cell Biologist
Roberta Gottlieb, SDSU Biology

Abstract

I will discuss some of the challenges we have had in developing therapies for ischemia/reperfusion injury. Cytochrome P450 inhibitors are potently cardioprotective and probably affect arachidonic acid metabolism, but is it through inhibition of EETs or HETEs?

What are the cellular targets of those metabolites? Could we develop some ligands to identify the enzyme responsible for arachidonic acid metabolism?

Tat-mediated protein transduction is a means to deliver recombinant proteins into cells and represents a novel therapeutic approach, but it is fraught with challenges in expression and purification of the recombinant fusion proteins.

What could we add to the buffer to keep the proteins from precipitating?

I may also talk about a hare-brained idea I would like to invite collaboration on, to develop a FRET parallel for NMR imaging.